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Måling af Osmotisk Vand permeabilitetskoefficienten (P<sub> F</sub>) Af sfæriske celler: Isolerede planteprotoplaster som et eksempel
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Biologia
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JoVE Journal Biologia
Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example

Måling af Osmotisk Vand permeabilitetskoefficienten (P<sub> F</sub>) Af sfæriske celler: Isolerede planteprotoplaster som et eksempel

DOI:
10.3791/51652-v

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14:20 min

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October 08, 2014

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, , , , ,
1The RH Smith Institute of Plant Sciences and Genetics in Agriculture,The Hebrew University of Jerusalem, 2Earth and Life Institute,Université catholique de Louvain, 3Institut des Sciences de la Vie,Université catholique de Louvain

Capitoli

  • 00:05Titolo
  • 02:10Isolation of Arabidopsis Mesophyll Protoplasts
  • 04:13The Hypotonic Challenge Assay: Arabidopsis Mesophyll Cell Swelling
  • 06:36Analysis of Cell Volume Change Using ImageJ
  • 08:12Modling the Rate of Osmolarity Change in the Experimental Chamber Using ImageJ and the Matlab Program PfFit
  • 11:11Determining the Pf Using the Matlab Fitting Program PfFit
  • 12:30Results: Determination of the Osmotic Water Permeability Coefficient (Pf) in Plant Protoplasts
  • 13:37Conclusion

Summary

Traduzione automatica

Traduzione automatica

Måling af osmotiske koefficient vandpermeabiliteten (P f) af celler kan hjælpe med at forstå de regulatoriske mekanismer aquaporiner (AQPs). P f beslutsomhed i sfæriske plantecelleprotoplaster præsenteres her involverer protoplaster isolation og numerisk analyse af deres oprindelige sats på volumen forandring som følge af en osmotisk udfordring under konstant bad perfusion.

Tags

Osmotic Water PermeabilityPlant ProtoplastsAQP RegulationCell Volume ChangesHypotonic SolutionOsmotic Challenge AssayPf Measurement

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