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Studying DNA Looping by Single-Molecule FRET
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Biologia
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JoVE Journal Biologia
Studying DNA Looping by Single-Molecule FRET

Studying DNA Looping by Single-Molecule FRET

DOI:
10.3791/51667-v

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11:27 min

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June 28, 2014

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1School of Physics,Georgia Institute of Technology

Capitoli

  • 00:05Titolo
  • 01:21Double Stranded (ds)DNA Sample Preparation
  • 02:52Flow Cell Preparation
  • 05:04Single-molecule Imaging
  • 06:18Image Processing and Data Analysis
  • 07:48Determining the J Factor
  • 09:44Results: Representative Analysis of DNA Looping
  • 10:52Conclusion

Summary

Traduzione automatica

Traduzione automatica

This study presents a detailed experimental procedure to measure looping dynamics of double-stranded DNA using single-molecule Fluorescence Resonance Energy Transfer (FRET). The protocol also describes how to extract the looping probability density called the J factor.

Tags

DNA LoopingFRETDNA BendingDNA-protein InteractionsDNA PackagingNucleosomeCyclizationDNA LigaseDNA StructureLooping KineticsPCRFRET PairBiotin LinkerJ FactorIntrinsic Curvature

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