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Collection and Extraction of Saliva DNA for Next Generation Sequencing
 

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Article DOI: 10.3791/51697-v • 06:58 min • August 27th, 2014
August 27th, 2014 •
1, 1, 1, 1,2, 1,3
1Battelle Center for Mathematical Medicine, The Research Institute at Nationwide Children's Hospital, 2The Interdisciplinary Graduate Program in Biophysics, The Ohio State University, 3Department of Pediatrics, The Ohio State University

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DNA extraction from saliva can provide a readily available source of high molecular weight DNA, with little to no degradation/fragmentation. This protocol provides optimized parameters for saliva collection/storage and DNA extraction to be of sufficient quality and quantity for downstream DNA assays with high quality requirements.

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Keywords: DNA Extraction Saliva Next-generation Sequencing DNA Collection DNA Quality DNA Quantity DNA Assays Microarray Genotyping
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