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A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium
DOI:

06:50 min

June 26, 2014

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Capitoli

  • 00:05Titolo
  • 00:53Preparations
  • 02:01Feeding RNAi to Nematodes
  • 03:34Treating C. elegans with Salmonella and Scoring Survival
  • 05:41C. elegans Survival from Salmonella Infection and bec-1
  • 06:25Conclusion

Summary

Traduzione automatica

C. elegans has emerged as a new genetic model to study host-pathogen interactions. Here we describe a protocol to infect C. elegans with Salmonella typhimurium coupled with the double-strand RNAi interference technique to examine the role of host genes in defense against Salmonella infection.

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