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Jsubtomo kullanılarak Elektron Cryotomography Rekonstrüksiyonunda ikinci viral zarf glikoproteini Spikes ortalamasını
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo
DOI:

08:29 min

October 21, 2014

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Capitoli

  • 00:05Titolo
  • 01:46Extracting Virus Sub‐volumes from Full‐size Tomograms
  • 02:32Generation of Two Independent Initial Models
  • 03:58Gold‐standard Iterative Alignment and Averaging of the Two Initial Spike Models & Generation and Alignment of Seeds to the Virus Surface for Template Matching
  • 05:48Gold‐standard Iterative Alignment and Averaging of the Spike Structure and Visualization of the Results
  • 06:44Results: Structure of the Envelope Glycoprotein complex of Bunyamwera Virus
  • 07:54Conclusion

Summary

Traduzione automatica

An approach is presented for determining structures of viral membrane glycoprotein complexes using a combination of electron cryo-tomography and sub-tomogram averaging with the computational package Jsubtomo.

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