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Analyzer celular baseado em impedância Real-Time como uma ferramenta para delinear vias moleculares envolvidas na neurotoxicidade e neuroproteção em uma linha de células neuronais
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line
DOI:

08:23 min

August 09, 2014

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Capitoli

  • 00:05Titolo
  • 01:39Plating and Proliferation of SK-N-SH Cells
  • 03:59Serum Deprivation
  • 05:04Data Analysis
  • 06:11Results: Representative Neurotoxicity/Neuroprotection Data
  • 07:53Conclusion

Summary

Traduzione automatica

Improved in vitro neurotoxicity assays would aid the identification of new neuroprotective compounds. The utility of a real-time impedance-based cell analyzer to determine cytotoxicity and cytoprotection in neuronal cell lines and to delineate the involvement of second messenger pathways, thus gaining insight in the mechanism of neuroprotection is presented.

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