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Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
 

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Article DOI: 10.3791/51764-v • 12:13 min • August 23rd, 2014
August 23rd, 2014 •
1, 1, 1, 1, 1
1Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University

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Reporter cell lines offer a means to visualize, track and isolate cells of interest from heterogeneous populations. However, gene-targeting using conventional homologous recombination in human embryonic stem cells is extremely inefficient. Herein, we describe targeting CNS midbrain specific transcription factor PITX3 locus with EGFP using zinc-finger nuclease enhanced homologous recombination.

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Keywords: Zinc-finger Nuclease Gene Targeting Human Embryonic Stem Cells Reporter Cell Lines PITX3 Parkinson's Disease Modeling Cell Replacement Therapy
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