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逆转录病毒感染的原发性肠化培养的视频协议
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
A Video Protocol of Retroviral Infection in Primary Intestinal Organoid Culture
DOI:

09:18 min

August 11, 2014

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Capitoli

  • 00:05Titolo
  • 01:17Crypt Isolation
  • 03:39Virus Production
  • 05:15Retroviral Transduction
  • 06:34Spinoculation and Plating
  • 08:01Results: Representative Organoid Images
  • 08:49Conclusion

Summary

Traduzione automatica

本协议解释主要LGR5-positve化培养和逆转录病毒转导的后续表现。这使Cre重组酶诱导表达递送转基因或敲除,并允许功能性研究,以进行在小说体外器官 模型系统

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