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Fluorescence Imaging with One-nanometer Accuracy (FIONA)
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Biologia
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JoVE Journal Biologia
Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

DOI:
10.3791/51774-v

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11:56 min

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September 26, 2014

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, , , , ,
1Department of Physics,University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells,University of Illinois at Urbana-Champaign, 3Center for Biophysics and Computational Biology,University of Illinois at Urbana-Champaign

Capitoli

  • 00:05Titolo
  • 01:11TIRM Setup
  • 04:40FIONA on Cy3-DNA
  • 06:28Data Analysis for FIONA on Cy3-DNA
  • 07:29Application of FIONA to Quantify Motor Dynamics at Nanometer Scale
  • 09:10Data Analysis and Finding the Step-size of Myosin Walking
  • 09:59Results: Single Fluorophores Are Localized with Nanometer Precision Using FIONA
  • 11:17Conclusion

Summary

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Single fluorophores can be localized with nanometer precision using FIONA. Here a summary of the FIONA technique is reported, and how to carry out FIONA experiments is described.

Tags

Fluorescence ImagingFIONASingle FluorophoresNanometer PrecisionTIRFMCy3-DNAMyosin Va MotorQuantum DotsSol-gelsRabbit Eye Corneas

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