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Capitoli
- 00:05Titolo
- 02:00Coverslip Cleaning
- 03:33Preparation of Calibration Samples
- 04:41Transfection
- 07:35Protein Labeling
- 08:58Image Acquisition
- 10:49Image Analysis
- 12:35Results: TIRF Microscopy Detects and Tracks Single Receptors on the Surface of Living Cells
- 14:31Conclusion
This protocol describes how to use total internal reflection fluorescence microscopy to visualize and track single receptors on the surface of living cells and thereby analyze receptor lateral mobility, size of receptor complexes as well as to visualize transient receptor-receptor interactions. This protocol can be extended to other membrane proteins.
