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A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders
 

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

Article DOI: 10.3791/51810-v • 11:48 min • June 8th, 2014
June 8th, 2014 •
1, 1, 1, 2, 1, 2, 3, 1,2
1Division of Rheumatology, Allergy and Clinical Immunology, University of California, Davis, 2Institute for Pediatric Regenerative Medicine, Shriners Hospitals for Children - Northern California, 3Department of Dermatology, University of California, Davis

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Differentiation of precursor cells into osteoclasts is regulated by cytokines and growth factors. Here, a novel gene transfer technique for differentiation of osteoclasts in vivo and cell culture protocols for differentiating precursor cells into osteoclasts in vitro as a method to study the effects of cytokines on osteoclastogenesis are described.

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Keywords: Osteoclasts Bone Resorption Musculoskeletal Disorders In Vivo Gene Transfer In Vitro Cell-based Assays Cytokines Osteoclastogenesis Osteoporosis Arthritis Drug Discovery
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