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Capitoli
- 00:05Titolo
- 01:04Sample Collection
- 01:46RNA Extraction Analysis
- 03:04Acrylamide Gel and Sample Preparation
- 03:58Electrophoretic Fractionation
- 04:41Gel Blotting
- 06:03Probe Synthesis, Membrane Crosslinking, and Prehybridization
- 07:27Membrane Hybridization, Washing, and Signal Detection
- 08:20Results: Improved RNA Detection Sensitivity
- 09:06Conclusion
The aim of this publication is to visualize and discuss the operative steps of an Enhanced Northern Blot protocol on RNA extracted from Drosophila melanogaster embryos, cells, and tissues. This protocol is particularly useful for the efficient detection of small RNA species.
