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Kombination Magnetic Sortering af Mother Celler og udsving Tests at analysere Genome ustabilitet ved Mitotisk Cell Aging i<em> Saccharomyces cerevisiae</em
JoVE Journal
Biologia
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JoVE Journal Biologia
Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae

Kombination Magnetic Sortering af Mother Celler og udsving Tests at analysere Genome ustabilitet ved Mitotisk Cell Aging i<em> Saccharomyces cerevisiae</em

DOI:
10.3791/51850-v

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11:08 min

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October 16, 2014

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,
1Department of Biological Sciences,Rensselaer Polytechnic Institute

Capitoli

  • 00:05Titolo
  • 01:57Determination of Mutation Rate and Frequency
  • 02:50Biotin Labeling
  • 04:44Bead Labeling and Magnetic Sorting
  • 07:25Determination of Replicative Age
  • 09:09Results: Mutation Frequencies and Rates in Yeast
  • 10:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

Mutationsrater hos unge Saccharomyces cerevisiae celler målt gennem udsvings- test bruges til at forudsige mutationsfrekvenser til mor celler i forskellige replikative aldre. Magnetisk sortering og flowcytometri anvendes derefter til måling af faktiske mutationsfrekvenser og alder mor celler for at identificere eventuelle afvigelser fra forudsagte mutationsfrekvenser.

Tags

Keywords: Saccharomyces CerevisiaeGenome InstabilityMitotic Cell AgingMagnetic SortingFluctuation TestsReplicative LifespanMother CellsDNA RepairDNA Damage ResponseMutation RatesMutation Frequencies

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