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Injection virale intracérébro du cerveau de souris nouveau-né pour la transduction neuronale persistante et généralisée
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction
DOI:

10:15 min

September 15, 2014

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Capitoli

  • 00:05Titolo
  • 01:24Staging the Newborn Pups for Injection
  • 02:38Free-hand Intracranial Injection of AAV into Neonatal Mice
  • 04:54Stereotaxic Injection of AAV into Neonatal Mice
  • 07:21Post-injection Care
  • 07:54Effect of Viral Titer and Serotype on VIral Transduction and Co-expression
  • 09:55Conclusion

Summary

Traduzione automatica

Here we demonstrate a technique for widespread neuronal transduction by intraventricular injection of adeno-associated virus into the neonatal mouse brain. This method provides a rapid and easy way to attain lifelong expression of virally-delivered transgenes.

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