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माउस मस्तिष्क के विभिन्न क्षेत्रों में टेलोमिरेज गतिविधि: गैर रेडियोधर्मी टेलोमिरेज प्रवर्धन प्रोटोकॉल दोहराएँ (ट्रैप) परख
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay
DOI:

10:14 min

September 02, 2014

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Capitoli

  • 00:05Titolo
  • 01:35Mouse Brain Protein Extraction
  • 03:25Telomeric Repeat Amplification Protocol (TRAP)
  • 06:12Agarose Mini-gel Electrophoresis and Gel Staining
  • 08:16Results: The Modified TRAP Assay is More Sensitive than the Traditional Assay in Detecting Telomerase Activity
  • 09:45Conclusion

Summary

Traduzione automatica

Telomerase is expressed in the neonatal brain and also in distinct regions of the adult brain. We present a non-toxic time saving TRAP assay for the analysis of telomerase activity in various regions of the mouse brain and detection of differences in telomerase activity between male and female mouse brains.

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