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Diseksiyon, Görüntüleme ve Farmakolojik Tedavi: Drosophila Pupa Testis ve Bekar Erkek germ-line Kistler ex vivo Kültür
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
DOI:

08:35 min

September 11, 2014

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Capitoli

  • 00:05Titolo
  • 01:12Dissection of Pupal Testes
  • 03:40Dissection of Male Germ-line Cysts and Live Imaging
  • 04:58Pharmacological Assay with Intact Pupal Testes
  • 06:30Results: Dynamic Analysis of Drosophila Testes
  • 08:08Conclusion

Summary

Traduzione automatica

This protocol describes the dissection and cultivation of intact testes and germ-line cysts from Drosophila melanogaster pupae. This method allows microscopic observation of spermatogenesis ex vivo. Furthermore, we describe a pharmacological assay of the effect of inhibitors on specific stages of germ-cell development in pupal testes.

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