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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
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Biologia
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JoVE Journal Biologia
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

DOI:
10.3791/51872-v

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08:07 min

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August 02, 2015

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, ,
1Department of Genetics,Stanford University, 2Stanford Center for Genomics and Personalized Medicine,Stanford University

Capitoli

  • 00:05Titolo
  • 02:06Probe Preparation
  • 05:03Probing the Arrays
  • 06:57Results: Microarray Studies Reveal a Protein-protein Interactions Between Tda-V5 and Rim11
  • 07:41Conclusion

Summary

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Using protein microarrays containing nearly the entire S. cerevisiae proteome is probed for rapid unbiased interrogation of thousands of protein-protein interactions in parallel. This method can be utilized for protein-small molecule, posttranslational modification, and other assays in high-throughput.

Tags

Protein MicroarrayProtein-protein InteractionsKinaseYeastAffinity PurificationV5-epitope TagRecombinant ProteinsHigh-density ArrayInformatics AnalysisIn Vivo Validation

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