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新方法本地化记者荧光珠近细胞培养表面的牵引力显微镜

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Bioingegneria

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JoVE Journal Bioingegneria

A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy

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新方法本地化记者荧光珠近细胞培养表面的牵引力显微镜

DOI:

10.3791/51873-v

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13:30 min

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September 16, 2014

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Samantha G. Knoll, M. Yakut Ali, M. Taher A. Saif

¹Department of Mechanical Science and Engineering,University of Illinois at Urbana-Champaign

Capitoli

00:05Titolo
01:42Functionalizing the Top Glass Cover Slips
03:18Preparing Polyacrylamide Gel Directly on Glass Bottom Petri Dishes
06:31Functionalizing Polyacrylamide Gel with Fibronectin
07:47Traction Force Experiments
10:22Results: Localizing Fluorescent Microspheres within Polyacrylamide Gels for Traction Force Microscopy
12:51Conclusion

Summary

Traduzione automatica

制造含有荧光探针聚丙烯酰胺（PA）的凝胶传统技术涉及的夹附着表面和玻璃载片之间的硅胶。这里，我们表明，涂敷该滑动用聚-D-赖氨酸（PDL）和荧光探针的局部化的探针内1.6微米的凝胶表面。

Tags

Keywords: Traction Force Microscopy PA Gels Fluorescent Beads Cell Traction Forces Surface Deformation Elasticity Theory Cell Adhesion Extracellular Matrix Surface Displacement Bead Localization Poly-D-lysine Gel Fabrication

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