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脳神経細胞の初代培養の確立が続く動物の急速なジェノタイピング
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons
DOI:

09:51 min

January 29, 2015

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Capitoli

  • 00:05Titolo
  • 01:33Fast PCR Genotyping of Newborn Mice
  • 02:56Primary Culture of Mouse Brain Neurons
  • 08:01Results: Representative Genotyping of Primary Neuronal Culture
  • 09:17Conclusion

Summary

Traduzione automatica

We describe procedures for labeling and genotyping newborn mice and generating primary neuronal cultures from them. The genotyping is rapid, efficient and reliable, and allows for automated nucleic-acid extraction. This is especially useful for neonatally lethal mice and their cultures that require prior completion of genotyping.

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