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Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines
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JoVE Journal Medicina
Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines

Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines

DOI:
10.3791/51891-v

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11:50 min

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September 10, 2014

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, , ,
1Department of Biochemistry and Molecular Biology,Indiana University School of Medicine

Capitoli

  • 00:05Titolo
  • 02:02Cell Culture and Fluorescent Labeling Ovarian Cancer Cell Lines
  • 03:39Enrichment for Cancer Stem Cells
  • 04:51CSC Characterization via Gene Expression Analysis for Stem Cell Markers
  • 06:10Analyzing CSCs for Cell Surface Markers CD117 and CD133
  • 07:42Analyzing CSCs for Therapeutic Response
  • 08:41Results: Validation of Ovarian CSCs Enrichment
  • 10:46Conclusion

Summary

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Traduzione automatica

Cancer stem cells (CSCs) are implicated in tumor relapse due to chemoresistance. We have optimized a protocol for selection and expansion of CSCs from ovarian cancer cell lines. By treating cells with the chemotherapeutic cisplatin and culturing in a stem cell promoting media we enrich for non-adherent CSC cultures.

Tags

Cancer Stem CellsChemoresistanceOvarian CancerSKOV3OVCA429CisplatinStem Cell MarkersOct4NanogProm1 (CD133)CD177CD133Tumor Relapse

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