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Bioengineering

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JoVE Journal Bioengineering
Luminescence resonansenergioverførsel at studere konformationsændringer i Membranproteiner udtrykkes i pattedyrceller
 
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Luminescence resonansenergioverførsel at studere konformationsændringer i Membranproteiner udtrykkes i pattedyrceller

Article DOI: 10.3791/51895-v • 08:31 min • September 16th, 2014
September 16th, 2014 •
1, 1, 1
1Center for Membrane Biology, Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston

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Vi beskriver her en forbedret Luminescence Resonance Energy Transfer (LRET) metode, hvor vi indfører en proteasespaltningssted mellem donor og acceptorfluoroforen websteder. Denne ændring giver os mulighed for at opnå konkrete LRET signaler, der stammer fra membranproteiner af interesse, der giver mulighed for studiet af membranproteiner uden proteinoprensning.

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Bioengineering LRET FRET Luminescence Resonance Energy Transfer fluorescensresonansenergioverførsel glutamatreceptorer syre sensing ionkanal protein konformation protein dynamik fluorescens protein-protein-interaktioner
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