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Capitoli
- 00:05Titolo
- 01:35N‐methyl‐N‐nitrosourea Treatment
- 02:35Visual Acuity Measurements
- 03:57Histology
- 05:12Immunohistochemstry
- 07:01Results: Visual Acuity Testing and Retinal Degeneration/Regeneration Analysis
- 08:16Conclusion
Herein we demonstrate quantification of retinal de- and regeneration and its impact on visual function using N-methyl-N-nitrosourea in the adult zebrafish. Loss of visual acuity and decreased photoreceptor numbers were followed by proliferation in the inner nuclear layer. Complete morphological and functional regeneration occurred 30 days after the initial treatment.
