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Proteinisolering fra udviklingslandene Embryonale Mouse hjerteklap Region
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Biologia
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JoVE Journal Biologia
Protein Isolation from the Developing Embryonic Mouse Heart Valve Region

Proteinisolering fra udviklingslandene Embryonale Mouse hjerteklap Region

DOI:
10.3791/51911-v

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06:55 min

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September 23, 2014

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, ,
1McAllister Heart Institute,University of North Carolina at Chapel Hill, 2New York-Presbyterian Hospital/Weill-Cornell Medical Center

Capitoli

  • 00:05Titolo
  • 01:00Aortic Valve Excision and Protein Extraction
  • 04:53Results: Representative Embryonic Aortic Valve Analyses
  • 06:24Conclusion

Summary

Traduzione automatica

Traduzione automatica

The analysis of protein expression in young embryonic mouse valves has been hampered by the limited tissue available. This manuscript provides a protocol for preparing protein from developing embryonic mouse valve regions for western blot analysis.

Tags

Protein IsolationEmbryonic Mouse Heart ValveWestern Blot AnalysisTris-based Lysis BufferProtease InhibitorsSDS-based Sample BufferSDS-PAGEPhosphorylated ProteinsCell Signaling PathwayEmbryonic Mouse Valve Development

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