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在髓系细胞动态实时成像<em>装甲运兵车<sup>最小/ +</sup</em>肠道肿瘤通过旋转盘共聚焦显微镜
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy
Please note that all translations are automatically generated. Click here for the English version.

在髓系细胞动态实时成像<em>装甲运兵车<sup>最小/ +</sup</em>肠道肿瘤通过旋转盘共聚焦显微镜

DOI:
10.3791/51916-v

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05:21 min

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October 06, 2014

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, ,
1Department of Oncology,INSERM U661, Functional Genomic Institute, 2Department of Anatomy,University of California

Capitoli

  • 00:05Titolo
  • 00:59Preparation of the Intestine for Imaging
  • 02:43Image Acquisition
  • 03:36Results: Representative Spinning Disk Confocal Images of Non-tumoral and Tumoral Tissues from ApcMin/+ Mice
  • 04:57Conclusion

Summary

Traduzione automatica

Traduzione automatica

By using transgenic reporter mice and injectable fluorescent labels, long-term intravital spinning disk confocal microscopy enables direct visualization of myeloid cell behavior into intestinal adenoma in the ApcMin/+ colorectal cancer model.

Tags

Keywords: Real-time ImagingMyeloid CellsIntestinal TumorsSpinning Disk Confocal MicroscopyApcMin/+ MiceC-fms-EGFPACTB-ECFPIntravital ImagingTumor MicroenvironmentIntestinal AdenomasBlood VesselsNeutrophils

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