Rivista
/
Biologia
/
Generation af<em> Enterobacter</em> Sp. YSU auxotrofer Brug Transposon Mutagenesis
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Generation af<em> Enterobacter</em> Sp. YSU auxotrofer Brug Transposon Mutagenesis

DOI:
10.3791/51934-v

•

13:31 min

•

October 31, 2014

•

1Department of Biological Sciences, Center for Applied Chemical Biology,Youngstown State University

Capitoli

  • 00:05Titolo
  • 02:27Electroporation of Competent Cells
  • 04:35Gridding Transformants and Replica Plating to Determine the Auxotroph Phenotype
  • 08:02Gene Rescue
  • 11:13Results: Generation of Auxotrophic Transformants in Enterobacter sp. YSU
  • 12:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

Enterobacter sp. YSU vokser i glucose minimale salte medium. Auxotrofer genereres ved at omdanne den med en transposome som tilfældigt indsætter sig selv i værtens genom. Mutanter findes ved replikaudpladning fra komplekst medium til minimalmedium. Afbrudte gener identificeres ved gen-redning og sekventering.

Tags

Keywords: Enterobacter Sp. YSUAuxotrophsTransposon MutagenesisTn5 TransposomeM-9 Minimal MediumR6K Gamma Replication OriginKanamycin ResistanceSanger SequencingBLASTGene Identification

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/51934?article_type=v

Generation of Stable Transgenic C. elegans Using Microinjection
check_url/it/51934?article_type=v

Homemade Site Directed Mutagenesis of Whole Plasmids
check_url/it/51934?article_type=v

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
check_url/it/51934?article_type=v

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
check_url/it/51934?article_type=v

An Introduction to Worm Lab: from Culturing Worms to Mutagenesis
check_url/it/51934?article_type=v

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
check_url/it/51934?article_type=v

Single oocyt hydrogensulfit Mutagenese
check_url/it/51934?article_type=v

Hurtig og effektiv zebrafisk Genotypebestemmelse Brug PCR med høj opløsning Melt Analyse
check_url/it/51934?article_type=v

Effektiv iPS Cell Generation fra Blood Brug episomer og HDAC-hæmmere
check_url/it/51934?article_type=v

En protokol for Funktionel vurdering af Whole-Protein Saturation mutagenese Biblioteker hjælp High-Throughput Sequencing

Read Article