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Real-time Cytotoxicity Assays in Human Whole Blood
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JoVE Journal Medicina
Real-time Cytotoxicity Assays in Human Whole Blood

Real-time Cytotoxicity Assays in Human Whole Blood

DOI:
10.3791/51941-v

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08:27 min

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November 07, 2014

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, , ,
1Research and Development,Adheren, Inc, 2Research and Development,Eureka Therapeutics

Capitoli

  • 00:05Titolo
  • 01:05Experimental Setup and Target Cell Preparation
  • 02:46Preparation of Cell Microarrays
  • 04:05Whole Blood Cytotoxicity Assays
  • 06:03Measuring Raji Cell Death via WCA
  • 07:55Conclusion

Summary

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The whole blood cytotoxicity assay (WCA) is a cytotoxicity assay developed by incorporating high-throughput cell positioning technology with fluorescence microscopy and automated image processing. Here, we describe how lymphoma cells treated with an anti-CD20 antibody can be analyzed real-time in human whole blood to provide quantitative cellular cytotoxicity analysis.

Tags

Keywords: Real-time Cytotoxicity AssayHuman Whole BloodCell-based AssayCell CytotoxicityTumor Cell PopulationsAntibody DrugsCell DeathCell KillingCell SurvivalTime-dependent CytotoxicityDose-dependent CytotoxicityLymphomaLeukemiaSolid TumorEx Vivo

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