È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:35Amplification of Plasmid Inserts and Addition of Poly T-tail by PCR
- 02:48In Vitro Transcription
- 03:59Treatment of Purified mRNA with Antartic Phosphatase
- 05:17mRNA Transfection of Cells
- 07:01Flow Cytometric Analyses of eGFP Expression in Cells
- 08:14Results: Modified mRNA is Expressed in Human Cells
- 09:34Conclusion
