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Journal / Medicine / Confocal Time Lapse Imaging as an Efficient…
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Medicine

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JoVE Journal Medicine
Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
 

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Article DOI: 10.3791/51949-v • 07:04 min • November 9th, 2014
November 9th, 2014 •
1, 1,2, 1,3, 1,2
1Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Université Lyon1, 2UFR d'Odontologie, Université Lyon1; Service de Consultations et de Traitements Dentaires, Hospices Civils de Lyon, 3UFR d'Odontologie, Université Paris Diderot; Service d'Odontologie, APHP, Hôpital Rothschild

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The most studied aspect of the biocompatibility of dental composites is their cytotoxicity 3D CLSM time lapse imaging combined with fluorescent signal quantification allows sensitive evaluation of the cell fate over time. Utilizing this method could be an efficient tool to assess the cytocompatibility of dental composites.

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Keywords: Confocal Time-lapse Imaging Cytocompatibility Dental Composites ELS Extra Low Shrinkage Primary Human Gingival Fibroblasts Live/Dead Staining FV10i Confocal System FV10-ASW 3.1 Software In Vitro Biocompatibility Assessment
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