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Immunologia e infezioni

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传播<em> Homalodisca coagulata病毒01</em>通过<em> Homalodisca vitripennis</em>细胞培养

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture

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传播<em> Homalodisca coagulata病毒01</em>通过<em> Homalodisca vitripennis</em>细胞培养

DOI:

10.3791/51953-v

•

12:52 min

•

September 25, 2014

•

Anna M. Biesbrock, Christopher M. Powell, Wayne B. Hunter, Blake R. Bextine

¹Department of Biology,University of Texas at Tyler, ²U. S. Horticultural Research Laboratory,USDA ARS

Capitoli

00:05Titolo
01:52Cell Culture and Whole Virus Extraction
03:56Viral Replication
04:55RNA Extraction and RT-PCR
07:45Confocal Microscopy
09:55Results: Quantification of Cell Survivability in HoCV-01 Infected Cells
12:03Conclusion

Summary

Traduzione automatica

在这里，我们提出了一个协议来传播Homalodisca vitripennis细胞和HoCV-1 体外。培养基从HoCV-1阳性培养去除和RNA的提取，每24小时为168小时。细胞生存力进行定量通过台盼蓝染色法。整个病毒粒子中提取的后感染。提取的RNA进行定量通过qRT-PCR。

Tags

Keywords: Homalodisca Vitripennis Homalodisca Coagulata Virus-01 (HoCV-01)Xylella Fastidiosa Pierce’s Disease Cell Culture Viral Propagation Biopesticide Production QRT-PCR Trypan Blue Confocal Microscopy

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