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Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection
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Capitoli
- 00:05Titolo
- 01:20Predifferentiation of THP-1 Macrophage and Preparation for Nucleofection
- 03:22Nucleofection of THP-1 Macrophage
- 04:53Post-Nucleofection Care
- 06:15Gross Characterization of siRNA Transfection THP-1 Macrophages
- 07:21Conclusion
This protocol presents an efficient and reliable method to transfect human THP-1 macrophages with siRNA or plasmid DNA by electroporation with high transfection efficiency while maintaining high cell vitality and full macrophage capacity for differentiation and polarization.
