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Préparation du Rat Serum Convient pour la culture d'embryons de mammifères entier
JoVE Journal
Biologia
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JoVE Journal Biologia
Preparation of Rat Serum Suitable for Mammalian Whole Embryo Culture
DOI:

11:48 min

August 03, 2014

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Capitoli

  • 00:00Titolo
  • 00:03Introduction
  • 01:49Blood Collection
  • 05:26Rat Serum Preparation
  • 07:43Risultati
  • 08:13Troubleshooting 1: Non-separation of Layers
  • 08:39Troubleshooting 2: Mild Hemolysis
  • 09:16Conclusion

Summary

Traduzione automatica

Mammalian whole embryo culture (WEC) is widely used in teratology and developmental biology. Immediately centrifuged rat serum is commonly provided as a medium for both mouse and rat WEC. In this video, we demonstrate our standard protocol for the preparation of high-quality rat serum suitable for mammalian WEC.

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