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Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos
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Neuroscienze
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JoVE Journal Neuroscienze
Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos

Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos

DOI:
10.3791/51991-v

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11:16 min

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September 24, 2014

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, ,
1Department of Natural Sciences,Assumption College

Capitoli

  • 00:05Titolo
  • 01:48Chick Dissection and Isolation of DRGs
  • 04:54Dissociation, Enriching, and Culturing DRG Neurons – Part 1
  • 07:29Dissociation, Enriching, and Culturing DRG Neurons – Part 2
  • 09:09Results: Isolation and Culture of Dissociated Sensory Neurons from Chick Embryos
  • 10:12Conclusion

Summary

Traduzione automatica

Traduzione automatica

Cell culture models provide detailed control over environmental conditions and thus provide a powerful platform to elucidate numerous aspects of neuronal cell biology. We describe a rapid, inexpensive, and reliable method to isolate, dissociate, and culture sensory neurons from chick embryos. Details of substrata preparation and immunocytochemistry are also provided.

Tags

Neuron IsolationCell CultureChick EmbryoSensory NeuronsPrimary NeuronsLaminin SubstrateIn Vitro ModelImmunocytochemistryLive Cell ImagingRNAi

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