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分离和游离感觉神经元的文化活动从鸡胚
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Neuroscienze
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JoVE Journal Neuroscienze
Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos
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分离和游离感觉神经元的文化活动从鸡胚

DOI:
10.3791/51991-v

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11:16 min

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September 24, 2014

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, ,
1Department of Natural Sciences,Assumption College

Capitoli

  • 00:05Titolo
  • 01:48Chick Dissection and Isolation of DRGs
  • 04:54Dissociation, Enriching, and Culturing DRG Neurons – Part 1
  • 07:29Dissociation, Enriching, and Culturing DRG Neurons – Part 2
  • 09:09Results: Isolation and Culture of Dissociated Sensory Neurons from Chick Embryos
  • 10:12Conclusion

Summary

Traduzione automatica

Traduzione automatica

细胞培养模型提供了环境条件的详细控制，从而提供一个强大的平台，以阐明神经元细胞生物学的许多方面。我们描述了一种快速，廉价和可靠的方法来从鸡胚分离，解离和培养的感觉神经元。还提供基质制剂和免疫细胞化学的细节。

Tags

Keywords: Neuron IsolationCell CultureChick EmbryoSensory NeuronsPrimary NeuronsLaminin SubstrateIn Vitro ModelImmunocytochemistryLive Cell ImagingRNAi

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