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单一平面照明模块和一个宽场显微镜微毛细管法
JoVE Journal
Ingegneria
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JoVE Journal Ingegneria
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
DOI:

08:53 min

August 15, 2014

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Capitoli

  • 00:05Titolo
  • 02:03Cell Spheroids Incubated with a Chemotherapeutic Drug
  • 02:55Light Sheet Adjustment
  • 04:09Cell Spheroid Application and Microscope Feed Synchronization
  • 05:31Measurement in Dynamic Liquid Environment and Data Acquisition and Analysis
  • 07:00Results: Imaging of Cell Spheroids Incubated with the Cytostatic Drug Doxorubicin
  • 08:07Conclusion

Summary

Traduzione automatica

为单一平面照明显微镜的模块(SPIM)中描述了很容易适应的倒宽视场显微镜和3维细胞培养物进行了优化。样品位于一个矩形的毛细管,并通过微流体系统的荧光染料,药物制剂或药物可以以小批量应用。

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