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在小鸡视网膜有效的基因转移的原代细胞培养研究：<em>前OVO</em>电穿孔法
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
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在小鸡视网膜有效的基因转移的原代细胞培养研究：<em>前OVO</em>电穿孔法

DOI:
10.3791/52002-v

•

08:59 min

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November 02, 2015

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, ,
1Wilmer Eye Institute,The Johns Hopkins University School of Medicine

Capitoli

  • 00:05Titolo
  • 02:00Preparation for Ex-ovo Electroporation
  • 04:00Collection of Retinal Cups from Chick Embryos
  • 05:40Electroporation
  • 07:24Results: Efficiency of Transgene Expression in Cultured Cells after Electroporation
  • 08:10Conclusion

Summary

Traduzione automatica

Traduzione automatica

鸡胚视网膜细胞培养物是宝贵的工具，用于感光生物学研究。我们已经开发了基于前OVO之前培养的视网膜质粒电高效的基因转移技术。这种技术显着地增加的转染效率比现有的协议，使遗传操作此系统是可行的。

Tags

Keywords: Chick RetinaPrimary Cell CultureEx-ovo ElectroporationPhotoreceptorGene TransferGenetic ManipulationTransfectionRNAiOverexpression

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