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Sekvens-specifik mærkning af nukleinsyrer og proteiner med methyltransferaser og cofaktor Analoger
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Biologia
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JoVE Journal Biologia
Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Sekvens-specifik mærkning af nukleinsyrer og proteiner med methyltransferaser og cofaktor Analoger

DOI:
10.3791/52014-v

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12:07 min

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November 22, 2014

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, , , ,
1Institute of Organic Chemistry, Department of Chemistry,RWTH Aachen University

Capitoli

  • 00:05Titolo
  • 02:26One-step DNA Labeling Using Aziridine Cofactors (SMIIing)
  • 05:07Two-step Protein Labeling Using Double-activated Cofactors (mTAG)
  • 08:41Results: Gel Analyses for SMILing and mTAG Reactions
  • 11:04Conclusion

Summary

Traduzione automatica

Traduzione automatica

DNA og proteiner sekvens-specifikt mærket med affinitet eller fluorescerende reportergrupper hjælp af DNA eller protein methyltransferaser og syntetiske cofaktor analoger. Afhængigt af cofaktor specificitet enzymer, aziridinen eller dobbelt aktiverede cofaktor analoger er ansat for en- eller to-trins mærkning.

Tags

MethyltransferasesS-Adenosyl-l-methionine (AdoMet Or SAM)DNA MethylationRNA MethylationProtein MethylationAziridine CofactorsSMILing DNAMTAGDouble-activated AdoMet AnaloguesHistone MethylationClick ChemistryDNA GenotypingMethylation Detection

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