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Kvantitativ immunofluorescensassay at måle variationen i proteinniveauer på centrosomer
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Biologia
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JoVE Journal Biologia
Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Kvantitativ immunofluorescensassay at måle variationen i proteinniveauer på centrosomer

DOI:
10.3791/52030-v

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09:39 min

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December 20, 2014

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,
1Department of Molecular Genetics,The Ohio State University

Capitoli

  • 00:05Titolo
  • 02:13Cell Growth and Treatment
  • 03:26Immunostaining
  • 06:28Immunofluorescence Imaging and Analysis: Centrosome Distance < 2 μm
  • 07:58Results: Centrosomal VDAC3 Regulated by Proteasome Mediated Degradation
  • 08:59Conclusion

Summary

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Here, a novel quantitative fluorescence assay is developed to measure changes in the level of a protein specifically at centrosomes by normalizing that protein’s fluorescence intensity to that of an appropriate internal standard.

Tags

Keywords: Quantitative ImmunofluorescenceProtein LevelsCentrosomesMitotic SpindlePrimary CiliaCell CycleCentriole AssemblyFluorescence MicroscopyVDAC3Proteasome-mediated Degradation

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