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白细胞亚群在暂时或永久的脑缺血模型体视学和流式细胞仪鉴定
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Stereological and Flow Cytometry Characterization of Leukocyte Subpopulations in Models of Transient or Permanent Cerebral Ischemia
DOI:

12:42 min

December 28, 2014

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Capitoli

  • 00:05Titolo
  • 02:20Common Carotid Artery and Distal Middle Cerebral Artery Occlusion by Ligation
  • 03:56Perfusion and Sectioning of Brain Tissue
  • 05:36Stereological Quantification of Infiltrated Neutrophils After Cerebral Ischemia by Optical Fractionation
  • 07:23Brain Dissociation and Single Cell Suspension Preparation
  • 09:41Results: Representative Flow Cytometric Analysis of Brain Leukocytes Obtained from Naïve, Sham and Ischemic Mice
  • 11:44Conclusion

Summary

Traduzione automatica

脑的骨髓细胞的表征下列行程可以通过体视用光学分馏方法来进行,或者通过大脑的流式细胞仪分析白细胞悬浮液。两者都是有用的技术来执行,在缺血性脑中发现的主要的骨髓细胞亚群的准确的表型区分。

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