Rivista
/
Medicina
/
Generation of Induced Pluripotent Stem Cells from Muscular Dystrophy Patients: Efficient Integration-free Reprogramming of Urine Derived Cells
JoVE Journal
Medicina
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Medicina
Generation of Induced Pluripotent Stem Cells from Muscular Dystrophy Patients: Efficient Integration-free Reprogramming of Urine Derived Cells

Generation of Induced Pluripotent Stem Cells from Muscular Dystrophy Patients: Efficient Integration-free Reprogramming of Urine Derived Cells

DOI:
10.3791/52032-v

•

09:11 min

•

January 28, 2015

•

,
1Department of Medicine,Medical College of Wisconsin

Capitoli

  • 00:05Titolo
  • 01:46Isolation and Expansion of Urine Cells
  • 04:06Urine Cell Reprogramming Using Sendai Virus
  • 05:30Urine Derived Induced Pluripotent Stem Cell (iPSC) Clonal Expansion
  • 06:53Results: Representative Generation of Viral Genome and Transgene Free iPSCs
  • 08:36Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol entails detailed procedures for isolation of urine derived cells from muscular dystrophy patients; their efficient and rapid reprogramming through Sendai virus transduction.

Tags

Keywords: Induced Pluripotent Stem CellsMuscular DystrophyUrine CellsReprogrammingSendai VirusCardiomyocytesDisease ModelingDrug Screening

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/52032?article_type=v

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
check_url/it/52032?article_type=v

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
check_url/it/52032?article_type=v

In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells
check_url/it/52032?article_type=v

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
check_url/it/52032?article_type=v

Generation of Prostate Cancer Patient Derived Xenograft Models from Circulating Tumor Cells
check_url/it/52032?article_type=v

Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
check_url/it/52032?article_type=v

Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus
check_url/it/52032?article_type=v

Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients
check_url/it/52032?article_type=v

Evaluation of Exon Inclusion Induced by Splice Switching Antisense Oligonucleotides in SMA Patient Fibroblasts
check_url/it/52032?article_type=v

Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid

Read Article