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Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
 

Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation

Article DOI: 10.3791/52053-v • 12:42 min • November 26th, 2014
November 26th, 2014 •
*1,2, *1,2, 1,2, 1, 1,2
1Division of Reproductive Sciences, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine
* These authors contributed equally

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We developed an easily customized strand-specific fluorescent in situ hybridization (FISH) protocol combined with immunofluorescence. This allows for a detailed examination of RNA dynamics with simultaneous insight into the chromatin structure, nuclear organization, and transcriptional regulation at the single cell level.

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Fluorescent In Situ Hybridization FISH Immunofluorescence Histone Modification Xist RNA X-chromosome Inactivation LncRNA-mediated Gene Silencing X-inactive Specific Xist Cloud Mammalian Females Chromatin-modifying Enzymes Epigenetic Landscapes Histone H3 Trimethyl-lysine 27 H3K27me3 Immuno-FISH Protocol Oligonucleotide Probes Riboprobes
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