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Osteoclast Derivation from Mouse Bone Marrow
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Biologia
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JoVE Journal Biologia
Osteoclast Derivation from Mouse Bone Marrow

Osteoclast Derivation from Mouse Bone Marrow

DOI:
10.3791/52056-v

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06:17 min

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November 06, 2014

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, , , , , , , , , , , , , ,
1Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery,Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine,Stanford University

Capitoli

  • 00:05Titolo
  • 01:28Media Preparation and Bone Marrow Isolation
  • 03:05Gradient Separation of Bone Marrow Cells and Cell Culturing
  • 04:57Results: Culturing Osteoclasts from Mouse Bone Marrow
  • 05:56Conclusion

Summary

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Osteoclasts are the principal bone-resorbing cell in the body. An ability to isolate osteoclasts in large numbers has resulted in significant advances in the understanding of osteoclast biology. In this protocol, we describe a method for isolation, cultivating and quantifying osteoclast activity in vitro.

Tags

OsteoclastBone MarrowMonocyte/macrophage LineageBone ResorptionBone RemodelingOsteoblastsSkeletal RemodelingBone MatrixBone FormationBone DiseaseOsteoporosisIn Vitro Osteoclast IsolationBone Remodeling CycleTherapeutic Strategies

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