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スライスこれホット:生理的温度における急性成人の脳スライス
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature
DOI:

08:46 min

October 30, 2014

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Capitoli

  • 00:05Titolo
  • 01:17Setting Up for the Slicing Procedure
  • 02:53Dissecting the Brain
  • 04:52Brain Slicing
  • 07:09Results: Patch-clamp Recordings from Cerebellar Neurons
  • 08:25Conclusion

Summary

Traduzione automatica

In this paper we show a method for preparing acute brain slices in physiological temperature, using a conventional physiological solution without special modifications for the cutting (such as adding sucrose) and without intracardial perfusion of the animal before slice preparation.

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