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Journal / Biology / A Guide to Modern Quantitative Fluorescent…
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A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies
 

A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies

Article DOI: 10.3791/52099-v • 11:01 min • November 20th, 2014
November 20th, 2014 •
1, 1, 2, 1, 1, 3,4, 2, 1,4
1Division of Neurobiology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, 2Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, 3Centre for Integrative Physiology, University of Edinburgh, 4Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh

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The advancement of western blotting using fluorescence has allowed detection of subtle changes in protein expression enabling quantitative analyses. Here we describe a robust methodology for detection of a range of proteins across a variety of species and tissue types. A strategy to overcome common technical problems is also provided.

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Keywords: Quantitative Fluorescent Western Blotting Western Blot Protein Detection Fluorescent Labels Comparative Expression Analysis Protein Quantification Troubleshooting Strategies
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