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Immunology and Infection

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JoVE Journal Immunology and Infection
Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells
 

Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells

Article DOI: 10.3791/52100-v • 10:02 min • December 12th, 2014
December 12th, 2014 •
1,2,3, 1,2,3, 1,2,3, 4, 1,2,3
1INSERM, U968, 2Sorbonne Universités, UPMC Paris 06, UMR_S 968, Institut de la Vision, 3CNRS, UMR_7210, 4Department of Biological Sciences, Center for Cancer, Genetic Diseases and Gene Regulation, Fordham University

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This article describes the protocol for the purification of photoreceptor outer segment fragments (POS) via ultracentrifugation from porcine/bovine retinae using homogenization and sucrose gradient centrifugation. This protocol allows the preparation of large stocks of POS aliquots, labeled or unlabeled, that can then be stored at -80 °C.

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Keywords: Photoreceptor Outer Segments Retinal Pigment Epithelial Cells Phagocytosis Assay Porcine Bovine Sucrose Gradient Photopigment FITC Labeling Fluorescence Quantification Oxidative Stress Aging
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