Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Live Imaging of Innate Immune and Preneoplastic…
JoVE Journal
Developmental Biology

This Contenuto is Open Access.

JoVE Journal Developmental Biology
Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin
 

Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin

Article DOI: 10.3791/52107-v • 08:52 min • February 3rd, 2015
February 3rd, 2015 •
1, 1, 1, 2, 1
1MRC Centre for Inflammation Research, Queen's Medical Research Institute, The University of Edinburgh, 2Department of Cell & Developmental Biology, University College London

Capitoli

Riepilogo
Automatic Translation

Studying the earliest events of preneoplastic cell progression and innate immune cell interaction is pivotal to understand and treat cancer. Here we describe a method to conditionally induce epithelial cell transformations and the subsequent live imaging of innate immune cell interaction with HRASG12V expressing skin cells in zebrafish larvae.

Tags

Live Imaging Innate Immune Cells Preneoplastic Cells Gal4/UAS Expression System Inducible System Zebrafish Larvae Epithelial Cell Transformation 4-Hydroxytamoxifen (4-OHT) KalTA4-ERT2/UAS System HRASG12V Expressing Skin Cells Temporal/spatial Resolution Trophic Function Of Innate Immunity Tumorigenesis Host Response Oncogenic Trophic Inflammatory Responses Ectopic Gene Expression
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter