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Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
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JoVE Journal Chimica
Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

DOI:
10.3791/52114-v

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08:37 min

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November 05, 2014

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, ,
1Department of Chemistry,Lehigh University

Capitoli

  • 00:05Titolo
  • 01:19PAD4 Transformation
  • 02:39PAD4 Expression
  • 03:58PAD4 Puification
  • 05:15PAD4 Inhibition Assay at Room Temperature
  • 06:37Results: PAD4 Assay Readings
  • 08:09Conclusion

Summary

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PAD4 is an enzyme responsible for the conversion of peptidyl-arginine to peptidyl-citrulline. Dysregulation of PAD4 has been implicated in a number of human diseases. A facile and high-throughput compatible fluorescence based PAD4 assay is described.

Tags

Fluorescence-based AssayPAD4 ActivityPro-fluorescence Substrate AnalogPost-translational ModificationsHistone TailsArginine To Citrulline ConversionHigh-throughput ScreeningCell LysatesPAD4 Over-expression

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