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Afledning af T-celler<em> In Vitro</em> Fra mus embryonale stamceller
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells

Afledning af T-celler<em> In Vitro</em> Fra mus embryonale stamceller

DOI:
10.3791/52119-v

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08:27 min

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October 14, 2014

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, , , , ,
1Department of Biological Sciences, Hunter College and Graduate Center,City University of New York, 2Sunnybrook Research Institute, Department of Immunology,University of Toronto

Capitoli

  • 00:05Titolo
  • 01:38Days 0 – 5: Initiation of Coculture and Trypsin-mediated Passage with Preplating
  • 03:55Day 8: Hematopoietic Progenitor Cell (HPC) Collection
  • 05:14Day 10 – 12: Media Change and No Trypsin Passage
  • 06:30Results: Representative Flow Cytometry Analyses at Specific time Points During mESC Differentiation In Vitro
  • 07:38Conclusion

Summary

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Traduzione automatica

Mouse embryonic stem cells can be differentiated to T cells in vitro using the OP9-DL1 co-culture system. Success in this procedure requires careful attention to reagent/cell maintenance, and key technique sensitive steps. Here we discuss these critical parameters and provide a detailed protocol to encourage adoption of this technology.

Tags

Keywords: Mouse Embryonic Stem CellsOP9/OP9-DL1 Co-culture SystemHematopoietic ProgenitorsHematopoiesisT Lymphocyte DifferentiationIn Vitro DifferentiationCell Culture MaintenanceProtocolTechnique Sensitive Steps

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