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Vurdering Leukocyt--endotel interaktioner Under strømningsforholdene i en<em> Ex vivo</em> Autoperfused Microflow Chamber Assay
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

Vurdering Leukocyt--endotel interaktioner Under strømningsforholdene i en<em> Ex vivo</em> Autoperfused Microflow Chamber Assay

DOI:
10.3791/52130-v

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09:01 min

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December 30, 2014

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, ,
1Angiogenesis Laboratory, Department of Ophthalmology,Harvard Medical School, Massachusetts Eye & Ear Infirmary, 2St. Elizabeth’s Medical Center

Chapters

  • 00:05Title
  • 01:19Coating the Chamber
  • 02:35Filling the Chamber
  • 03:41Connecting the Chamber to the Mouse
  • 06:04Recording Rolling Velocity
  • 07:32Results: Representative Time Course of Leukocytes Rolling Through the Flow Chamber
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi en protokol, der tillader investigator at vurdere leukocytrekruttering dynamik ex vivo ved at forbinde et kammer belagt med endotel-afledt adhæsionsmolekyler til kredsløbssystemet på en mus. Denne metode giver betydelige fordele, da det giver mulighed for leukocyt vurdering efter relative biologiske forhold.

Tags

Leukocyte-endothelial InteractionsEx Vivo Autoperfused Microflow Chamber AssayInflammationPeripheral Blood Leukocytes (PBLs)Adhesion MoleculesIntravital MicroscopyFlow ConditionsLeukocyte Rolling VelocityImageJ

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