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Polikistik Böbrek Hastalığı Olası Zebra balığı Modeli: demonte<em> Wnt5a</em> Zebra balığı Böbrekler Kistler Nedenler
JoVE Journal
Medicina
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JoVE Journal Medicina
A Possible Zebrafish Model of Polycystic Kidney Disease: Knockdown of wnt5a Causes Cysts in Zebrafish Kidneys
DOI:

10:51 min

December 02, 2014

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Capitoli

  • 00:05Titolo
  • 01:46Preparation of Morpholino and Injection Apparatus
  • 03:44Preparation of Fertilized Zebrafish Embryos and Injection with Morpholinos
  • 04:55Phenotype Rescue Experiments
  • 06:42Florescent Imaging of Injected Embryos
  • 07:55Results: Analysis of wnt5a Morpholino Knockdown in Zebrafish
  • 10:09Conclusion

Summary

Traduzione automatica

We describe a method of generating a possible zebrafish model of polycystic kidney disease. We used Tg(wt1b:GFP) fish to visualize kidney structure. Knockdown of wnt5a was by morpholino injection. Pronephric cyst formation after wnt5a knockdown was observed in this GFP transgenic zebrafish.

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