Rivista
/
Immunologia e infezioni
/
T细胞受体删除环同时定量（TRECs）和K-删除重组删除环（KRECs）通过实时PCR
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR
Please note that all translations are automatically generated. Click here for the English version.

T细胞受体删除环同时定量（TRECs）和K-删除重组删除环（KRECs）通过实时PCR

DOI:
10.3791/52184-v

•

14:14 min

•

December 06, 2014

•

, , , , , ,
1CREA, Diagnostics Department,Spedali Civili di Brescia

Capitoli

  • 00:05Titolo
  • 02:34Preparation of Triple-insert Plasmid
  • 04:49Standard Curve Preparation and Real Time PCR for Quantification of TRECS and KRECS
  • 07:25Calculations
  • 11:59Results: TREC and KREC Quantification in Healthy Individuals
  • 12:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we describe a method for simultaneous quantification of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs). The TREC/KREC assay can be used as marker of thymic and bone marrow output.

Tags

Keywords: T-cell Receptor Excision Circles (TRECs)K-deleting Recombination Excision Circles (KRECs)Real-time PCRSimultaneous QuantificationImmune ReconstitutionBone Marrow TransplantationImmune DeficienciesImmunomodulating Drugs

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/52184?article_type=v

与预先定义的T细胞受体特异性Transnuclear小鼠对弓形虫获得通过体细胞核移植
check_url/it/52184?article_type=v

一个实时，同步成像的TCR和其相关的信号转导蛋白的T​​IRF显微镜技术
check_url/it/52184?article_type=v

小胶质细胞的小分子RNA检测在正常中枢神经系统的实时PCR技术在神经发炎
check_url/it/52184?article_type=v

T细胞实时成像实时信号复合物的形成
check_url/it/52184?article_type=v

在髓系细胞动态实时成像<em>装甲运兵车<sup>最小/ +</sup</em>肠道肿瘤通过旋转盘共聚焦显微镜
check_url/it/52184?article_type=v

高通量实时定量RT-PCR检测方法的确定I型和III型干扰素亚型的表达谱
check_url/it/52184?article_type=v

骨髓祖细胞的逆转录病毒转导到生成T细胞受体Retrogenic小鼠
check_url/it/52184?article_type=v

基因分型<em>金黄色葡萄球菌</em>由核糖体垫片的PCR（RS-PCR）
check_url/it/52184?article_type=v

用两相循环流系统实时成像和定量真菌生物膜的研究
check_url/it/52184?article_type=v

化学抑制剂库筛选 t 细胞受体信号的中介识别

Read Article